Di Matteo, who has been in charge of Schalke since October 2014, when he replaced manager Jens Keller, led the club to the Champions League round of 16 this season before falling to defending champions Real Madrid 5-4 on aggregate, despite a 4-3 second leg win at the Bernabeu.
Di Matteo will always be remembered for his heroics with Chelsea as he lifted the Champions League with the Blues a feat even Jose Mourihno has not been able to achieve.
After having what can be described as a good season in Europe the Royals Blues could not replicate the same on the domestic font as they failed to secure a place in the UEFA Champions League for next season to make matters worst
and on the final match day of the season went down 2-0 at Hamburg. The result kept the hosts in the league, while Schalke dropped down to sixth in the standings.
While the Royal Blues have qualified for the Europa League, they could still be forced into the playoffs for the smaller European competition should local rivals Borussia Dortmund win the German Cup against VfL Wolfsburg next week.
Schalke won just five of their 17 matches in the second half of the season, and following their last win against relegated SC Paderborn last week, the fans turned against the club and called for the board to quit.
Earlier this month, in an attempt to turn the season around, former AC Milan midfielder Kevin-Prince Boateng and former Germany international Sidney Sam suspended indefinitely by the club's sporting executive Horst Heldt.
Following the 2-0 loss at Hamburg on Saturday, Heldt announced on German TV he'd "question every position" at the club. "That's clear. All, who are responsible for the sporting side, be it the coach, the players or the sporting executive. Everything has to be put on the table, and then we'll make a decision."
Heldt, who has been at the club since June 2010, is also in danger of losing his job.
The loss on Saturday against Hamburg was the last nail on the coffin for Roberto as he had to be kicked out to get back the hearts of the already angry fans.